NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. • NHS pre-activated resin for coupling of small amino-containing proteins and peptides in process-scale applications • High level of pre-activation gives a high degree of substitution of the selected ligand • NHS coupling method results in chemically stable ligand attachment • BioProcess resin supported for industrial applications.

Solutions & buffers Step 1 Activation buffer. Dilute 4 μl HCl in 50 ml water for a final concentration of 1 mM Coupling buffer.

Component Final concentration Stock Amount NaHCO 3, pH 8.3 100 mM 1 M 100 ml NaCl 500 mM 5 M 100 ml Add water to 1 l Step 3 Quenching buffer. Dilute 5 ml of 1 M Tris–HCl, pH 8.0 in 45 ml water for a final concentration of 100 Mm Step 4 High pH wash buffer. Component Final concentration Stock Amount Tris–HCl, pH 8.0 100 mM 1 M 25 ml NaCl 500 mM 5 M 25 ml Add water to 250 ml Low pH wash buffer. Component Final concentration Stock Amount NaOAc, pH 4.0 100 mM 1 M 25 ml NaCl 500 mM 5 M 25 ml Add water to 250 ml Storage buffer (PBS, pH 7.4) Component Final concentration Stock Amount NaCl 137 mM 5 M 1.37 ml KCl 2.7 mM 4 M 33.8 μl NaH 2PO 4 10 mM 0.5 M 1 ml K 2HPO 4 2 mM 0.5 M 0.2 ml Add water to 50 ml.

Sepharose High Performance is the base matrix for HiTrap NHS-activated HP. Abdul rauf roofi naat allah karam mp3 download 2017. The carbohydrate nature of the agarose base provides a hydrophilic and chemically favorable environment for coupling, while the highly cross-linked structure of the 34-µm spherical matrix ensures excellent chromatographic properties. GE Healthcare NHS-Activated Sepharose™ 4 Fast Flow Affinity Media. Enet usb20 jpeg web camera driver. Use for coupling amino-containing smaller proteins and peptides. GE Healthcare Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH. This product(s) resides on a Fisher Scientific GSA or VA contract.